Yoshizaki K, Nakagawa T, Kaieda T, Muraguchi A, Yamamura Y, Kishimoto T
J Immunol. 1982 Mar;128(3):1296-301.
The proliferation and differentiation of human leukemic B cells (B-CLL cells) with anti-Ig and T cell-derived helper factors are described. Stimulation of B-CLL cells with anti-Ig and T helper factors could induce proliferation as well as differentiation into IgM- and IgG-producing cells. Neither anti-Ig nor T helper factors alone could induce any proliferation and/or differentiation of B-CLL cells. Not only whole molecules of anti-Ig but also F(ab')2 fragments could induce proliferation and differentiation of B-CLL cells in the presence of T helper factors, but monovalent Fab' fragments were not effective. Induction of both IgM and IgG with the same idiotype was confirmed by immunofluorescent and SDS-PAGE analysis. By employing an IL 2-dependent cytotoxic T cell line and a TRF-responsive B cell line, T cell factors were separated into a fraction with IL2 activity but no TRF activity and a fraction with TRF activity but no IL 2 activity by chromatofocusing. Anti-Ig and IL 2 fraction could induce proliferation of B-CLL cells, but TRF fraction was not effective for the induction of proliferation in anti-IG-stimulated cells. For IgM and IgG production, anti-Ig and both IL 2 and TRF fractions were required. Depletion of IL 2 fraction in the first 2 days' culture inhibited Ig production, whereas the absence of TRF fraction in the first 2 days did not show any inhibitory effect on Ig production.
本文描述了人白血病B细胞（B-CLL细胞）在抗Ig和T细胞衍生辅助因子作用下的增殖和分化情况。用抗Ig和T辅助因子刺激B-CLL细胞可诱导其增殖，并分化为产生IgM和IgG的细胞。单独的抗Ig或T辅助因子均不能诱导B-CLL细胞的任何增殖和/或分化。在T辅助因子存在的情况下，不仅抗Ig的完整分子，而且F(ab')2片段均可诱导B-CLL细胞的增殖和分化，但单价Fab'片段无效。通过免疫荧光和SDS-PAGE分析证实了相同独特型的IgM和IgG的诱导。利用白细胞介素2（IL 2）依赖的细胞毒性T细胞系和TRF反应性B细胞系，通过色谱聚焦将T细胞因子分离为具有IL2活性但无TRF活性的部分和具有TRF活性但无IL 2活性的部分。抗Ig和IL 2部分可诱导B-CLL细胞增殖，但TRF部分对诱导抗IG刺激细胞的增殖无效。对于IgM和IgG的产生，需要抗Ig以及IL 2和TRF部分。培养前两天IL 2部分的缺失会抑制Ig的产生，而前两天TRF部分的缺失对Ig的产生未显示任何抑制作用。
