Thomsen A R, Bro-Jørgensen K, Volkert M
Scand J Immunol. 1983 Feb;17(2):139-45. doi: 10.1111/j.1365-3083.1983.tb00776.x.
Fatal meningitis following intracerebral inoculation of lymphocytic choriomeningitis virus (LCMV) reflects an immunopathological lesion believed to be mediated by cytotoxic T cells. The results presented here demonstrate that pretreatment with cyclophosphamide (Cy; 150 mg/kg body weight) 2 days before intracerebral infection significantly reduced the lethality of the infection. However, this treatment did not impair the antiviral cytotoxic response as measured in the spleen. On the other hand, virus-specific delayed-type hypersensitivity (DTH) was significantly reduced. This reduction seems to be the result of a Cy-induced lack of non-committed ancillary cells since: (1) virus-primed spleen cells from Cy-pretreated donors conferred normal LCMV-specific DTH to naive recipients; (2) transfer of virus-primed spleen cells from untreated donors did not increase the suppressed DTH response of the Cy-pretreated mice; and (3) inoculation of irrelevant antigen and antigen-primed spleen cells into the footpads of Cy-pretreated, infected mice resulted in a significantly reduced footpad swelling as compared with untreated, infected controls. Taken together, these results indicate that LCMV-induced meningitis does not solely represent T-cell-mediated cytotoxicity in vivo but that a fatal outcome of the infection critically involves not only effector T cells but also ancillary cells.
脑内接种淋巴细胞性脉络丛脑膜炎病毒（LCMV）后发生的致命性脑膜炎反映了一种免疫病理损伤，据信是由细胞毒性T细胞介导的。本文给出的结果表明，在脑内感染前2天用环磷酰胺（Cy；150mg/kg体重）进行预处理可显著降低感染的致死率。然而，这种处理并未损害在脾脏中检测到的抗病毒细胞毒性反应。另一方面，病毒特异性迟发型超敏反应（DTH）显著降低。这种降低似乎是Cy诱导的未分化辅助细胞缺乏的结果，因为：（1）来自经Cy预处理供体的病毒致敏脾细胞赋予未致敏受体正常的LCMV特异性DTH；（2）从未经处理供体转移病毒致敏脾细胞并未增加经Cy预处理小鼠受抑制的DTH反应；（3）与未处理的感染对照相比，将无关抗原和抗原致敏脾细胞接种到经Cy预处理的感染小鼠脚垫中导致脚垫肿胀显著减轻。综上所述，这些结果表明，LCMV诱导的脑膜炎在体内并非仅代表T细胞介导的细胞毒性，而且感染的致命结局不仅严重涉及效应T细胞，还涉及辅助细胞。
