Gautam S, Deodhar S D
J Natl Cancer Inst. 1983 May;70(5):923-30.
The present study was performed to identify the subsets of oncofetal antigen-induced T-cells that mediate antitumor immune response in mice. In this study, performed on a weakly immunogenic, metastatic tumor, fetal antigen-sensitized spleen cells significantly inhibited the growth and metastases of tumors in Winn neutralization assay. Mixing of spleen cells from tumor-bearing inbred C57BL/6J mice with fetal antigen-sensitized spleen cells completely abolished the tumor inhibitory effect of fetal antigen-sensitized spleen cells. Further characterization of fetal antigen-sensitized spleen cells showed that they were Thy-1+. These findings reveal that tumor-associated fetal antigens on fetal cells can produce a tumor inhibitory T-cell immune response against the growth and metastases of a weakly immunogenic, metastatic tumor. The subset of oncofetal antigen-induced T-cells that mediates antitumor immune response was identified as Lyt-1+ and Lyt-2-. Adoptive transfer of fetal antigen-sensitized spleen cells to mice with preestablished metastases significantly inhibited the lung metastases in these mice, thus making this approach more relevant to a clinical situation in cancer patients.
本研究旨在鉴定癌胚抗原诱导的介导小鼠抗肿瘤免疫反应的T细胞亚群。在这项针对弱免疫原性转移性肿瘤进行的研究中，胎儿抗原致敏的脾细胞在Winn中和试验中显著抑制了肿瘤的生长和转移。将荷瘤近交系C57BL/6J小鼠的脾细胞与胎儿抗原致敏的脾细胞混合，完全消除了胎儿抗原致敏脾细胞的肿瘤抑制作用。对胎儿抗原致敏脾细胞的进一步表征显示它们是Thy-1+。这些发现表明，胎儿细胞上的肿瘤相关胎儿抗原可产生针对弱免疫原性转移性肿瘤生长和转移的肿瘤抑制性T细胞免疫反应。介导抗肿瘤免疫反应的癌胚抗原诱导的T细胞亚群被鉴定为Lyt-1+和Lyt-2-。将胎儿抗原致敏的脾细胞过继转移到已发生转移的小鼠中，显著抑制了这些小鼠的肺转移，从而使该方法与癌症患者的临床情况更相关。
