Wiman B, Nilsson T
Clin Chim Acta. 1983 Mar 14;128(2-3):359-66. doi: 10.1016/0009-8981(83)90335-2.
A convenient method for the determination of C1-esterase inhibitor activity in plasma samples is described. The method is based on addition of purified C1s to plasma and measuring excess C1s with a new chromogenic tripeptide-p-nitroanilide substrate with a recording spectrophotometer. Addition of C1s to C1-esterase inhibitor-depleted plasma did not result in any appreciable inactivation of the enzyme for three hours. The concentration of C1-esterase inhibitor in 19 healthy individuals was estimated as 1.63 +/- 0.27 (SD) mumol/l. The correlation with C1-esterase inhibitor antigen in these individuals and 19 patients with varying concentrations of C1-esterase inhibitor was excellent. The correlation with an antikallikrein assay was found to be poor.
本文描述了一种测定血浆样本中C1酯酶抑制剂活性的简便方法。该方法基于向血浆中添加纯化的C1s，并使用新型发色三肽 - 对硝基苯胺底物通过记录分光光度计测量过量的C1s。向去除C1酯酶抑制剂的血浆中添加C1s，三小时内酶没有出现任何明显的失活。19名健康个体中C1酯酶抑制剂的浓度估计为1.63±0.27（标准差）μmol/L。这些个体与19名C1酯酶抑制剂浓度不同的患者中C1酯酶抑制剂与抗原的相关性非常好。发现与抗激肽释放酶测定的相关性较差。
