Trafford D J, Makin H L
Clin Chim Acta. 1983 Mar 28;129(1):19-25. doi: 10.1016/0009-8981(83)90147-x.
Saliva collected from volunteers of both sexes at various times throughout the day was examined for the presence of 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3. The method used was a specific high-performance liquid chromatographic procedure with a minimum detectable limit for 25-hydroxyvitamin D of 0.05 microgram/1, (130 pmol/1 for 25-hydroxyvitamin D3) using 20 ml of saliva. No 25-hydroxyvitamin D could be detected in any of the samples. Examination of larger quantities of saliva (100 ml) by a combination of high-performance liquid chromatography and gas chromatography-mass spectrometry showed the presence of a peak possibly derived from 25-hydroxyvitamin D3, at a concentration not in excess of 65 pmol/l. Using known binding characteristics of 25-hydroxyvitamin D and plasma vitamin D binding protein, and comparing them with similar characteristics for cortisol and plasma cortisol binding globulin and the known salivary concentration of cortisol, simple calculations indicated that a likely concentration of 25-hydroxyvitamin D in saliva would be around 8 pmol/l. Previously reported values ranging from 130-4160 pmol/l (50-1600 micrograms/l) using a simple competitive protein binding assay could not be confirmed.
收集了不同性别的志愿者在一天中不同时间的唾液，检测其中25-羟基维生素D2和25-羟基维生素D3的存在情况。所采用的方法是一种特定的高效液相色谱法，使用20毫升唾液时，25-羟基维生素D的最低检测限为0.05微克/升（25-羟基维生素D3为130皮摩尔/升）。在任何样本中均未检测到25-羟基维生素D。通过高效液相色谱法和气相色谱-质谱联用对大量唾液（100毫升）进行检测，结果显示存在一个可能源自25-羟基维生素D3的峰，其浓度不超过65皮摩尔/升。利用25-羟基维生素D和血浆维生素D结合蛋白的已知结合特性，并将其与皮质醇和血浆皮质醇结合球蛋白的类似特性以及已知的唾液皮质醇浓度进行比较，简单计算表明唾液中25-羟基维生素D的可能浓度约为8皮摩尔/升。此前使用简单竞争性蛋白结合测定法报告的130 - 4160皮摩尔/升（50 - 1600微克/升）的值无法得到证实。
