Mawer E B, Hann J T
J Chromatogr. 1987 Apr 10;415(2):305-16. doi: 10.1016/s0378-4347(00)83222-3.
A system is described using high-performance liquid chromatography to separate and quantify, by spectrophotometry in a simple one-stage procedure, ercalcidiol (25-hydroxyvitamin D2) and calcidiol (25-hydroxyvitamin D3). The novel feature of the method is the employment of an ultraviolet-absorbing internal standard to monitor recovery. This has the advantage of permitting total automation of the quantification by eliminating the need for radioactivity counting. The method gives results that compare well with those obtained in other systems and has particular application in clinical studies where rapid separate determination of ercalcidiol and calcidiol is required.
本文描述了一种使用高效液相色谱法的系统，该系统通过简单的一步分光光度法程序来分离和定量骨化二醇（25-羟基维生素D2）和骨化二醇（25-羟基维生素D3）。该方法的新颖之处在于使用了一种紫外线吸收内标来监测回收率。这具有通过消除放射性计数的需要而允许定量完全自动化的优点。该方法给出的结果与其他系统获得的结果相当，并且在需要快速分别测定骨化二醇和骨化二醇的临床研究中有特殊应用。
