原始造血祖细胞在无血清培养基中的集落形成。
Colony formation by primitive hemopoietic progenitor cells in serum-free medium.
作者信息
Eliason J F, Odartchenko N
出版信息
Proc Natl Acad Sci U S A. 1985 Feb;82(3):775-9. doi: 10.1073/pnas.82.3.775.
A serum-free medium has been developed for clonal growth of murine hemopoietic progenitor cells. In this medium, the number of nonerythroid colonies induced by factors produced by cloned T lymphocytes was 90 +/- 10% of the number generated in serum-containing medium. Erythroid colony number in serum-free cultures containing the T-cell factors but no exogenous erythropoietin was significantly higher than that in cultures with serum, and the cloning efficiency was independent of cell concentration. Further addition of erythropoietin increased erythroid colony number approximately equal to 4-fold. Pure erythropoietin alone stimulated erythroid colony formation, but the cloning efficiency was highly dependent on cell concentration. Analysis of individual colonies generated in serum-free cultures containing the T-cell factors indicated that some contained cells of several hemopoietic lineages, demonstrating that multipotential progenitors can give rise to colonies in this system.
已开发出一种用于小鼠造血祖细胞克隆生长的无血清培养基。在这种培养基中，克隆的T淋巴细胞产生的因子诱导的非红系集落数量是含血清培养基中产生数量的90±10%。在含有T细胞因子但无外源性促红细胞生成素的无血清培养物中，红系集落数量显著高于含血清的培养物，且克隆效率与细胞浓度无关。进一步添加促红细胞生成素可使红系集落数量增加约4倍。单独的纯促红细胞生成素可刺激红系集落形成，但克隆效率高度依赖于细胞浓度。对含有T细胞因子的无血清培养物中产生的单个集落进行分析表明，一些集落包含几种造血谱系的细胞，这表明多能祖细胞可在该系统中形成集落。
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