血红蛋白A和S对人红细胞血影的影响。

Effect of hemoglobin A and S on human erythrocyte ghosts.

作者信息

Wiedenmann B, Elbaum D

出版信息

J Biol Chem. 1983 May 10;258(9):5483-9.

Abstract

The interaction of erythrocyte ghosts and vesicles with chromatographed hemoglobin (Hb) A and Hb S was studied under various conditions. Although no binding of either Hb A or Hb S to inside-out vesicles was detected, under conditions of physiological ionic strength and pH, several properties of white membrane ghosts were effected by the presence of Hb. Addition of Hb A and Hb S (2 g/dl) to membrane ghosts in 6 mM MgATP, 150 mM NaCl, 10 mM Tris-HCl buffer, pH 7.4, was found to effect the echinocyte-discocyte transition, the extent of endocytosis, the volume, and the sealing of ghosts. Our observations suggest that the structure of membrane ghosts is influenced by cytosol proteins and that the environment of the red cell membrane plays an important role in the definition and the control of the membrane structure and function.

摘要

在各种条件下研究了红细胞血影和囊泡与经色谱分离的血红蛋白（Hb）A和Hb S的相互作用。尽管未检测到Hb A或Hb S与外翻囊泡的结合，但在生理离子强度和pH条件下，Hb的存在影响了白色膜血影的几个特性。发现在6 mM MgATP、150 mM NaCl、10 mM Tris-HCl缓冲液（pH 7.4）中向膜血影添加Hb A和Hb S（2 g/dl）会影响棘状红细胞-盘状红细胞转变、内吞作用程度、体积以及血影的封闭性。我们的观察结果表明，膜血影的结构受胞质溶胶蛋白影响，并且红细胞膜的环境在膜结构和功能的定义及控制中起重要作用。