镰状细胞血红蛋白与红细胞膜的相互作用。
Interaction of sickle cell hemoglobin with erythrocyte membranes.
作者信息
Shaklai N, Sharma V S, Ranney H M
出版信息
Proc Natl Acad Sci U S A. 1981 Jan;78(1):65-8. doi: 10.1073/pnas.78.1.65.
The interactions of hemoglobin S with the erythrocyte membrane were compared with the corresponding interactions of hemoglobin A by measuring in both steady-state and kinetic experiments the quenching of the fluorescence of a probe embedded in erythrocyte membranes. Whereas hemoglobin A could be dissociated from membranes, a fraction of hemoglobin S was irreversibly bound even in the oxy state. Deoxyhemoglobin S interacted much more strongly with erythrocyte membranes than did deoxyhemoglobin A: a portion of the deoxyhemoglobin S was irreversibly bound, and the reversibly bound fraction of hemoglobin S dissociated more slowly than did deoxyhemoglobin A. It is suggested that the binding of deoxyhemoglobin S is a two-step reaction in which the first step involves electrostatic interaction with band III erythrocyte membrane protein and the second step involves a hydrophobic interaction with membrane lipids. The latter reaction reflects the greater hydrophobicity of hemoglobin S. The unique interaction of hemoglobin S with erythrocyte membranes may be important in the formation of irreversibly sickled cells.
通过在稳态和动力学实验中测量嵌入红细胞膜中的探针的荧光猝灭，比较了血红蛋白S与红细胞膜的相互作用以及血红蛋白A的相应相互作用。虽然血红蛋白A可以从膜上解离，但即使在氧合状态下，仍有一部分血红蛋白S不可逆地结合。脱氧血红蛋白S与红细胞膜的相互作用比脱氧血红蛋白A强得多：一部分脱氧血红蛋白S不可逆地结合，并且血红蛋白S的可逆结合部分比脱氧血红蛋白A解离得更慢。有人提出，脱氧血红蛋白S的结合是一个两步反应，第一步涉及与带III红细胞膜蛋白的静电相互作用，第二步涉及与膜脂质的疏水相互作用。后一反应反映了血红蛋白S更大的疏水性。血红蛋白S与红细胞膜的独特相互作用可能在不可逆镰状细胞的形成中起重要作用。
Zotero 插件