Claas F H, van Steenbrugge G J
Tissue Antigens. 1983 Mar;21(3):227-32. doi: 10.1111/j.1399-0039.1983.tb00162.x.
A permanent human tumor-line PC-93 was HLA allotyped with the use of a complement-dependent cytotoxicity test supplemented with absorption experiments. In comparison with the peripheral blood lymphocytes of the patient, whose adenocarcinoma of the prostate was the origin of PC-93, remarkable differences were observed. The tumor line lacked the expression of the genetically appropriate HLA-A1, A2 and B15 antigens. Instead of these antigens, cross-reactive HLA antigens seemed to be expressed on PC-93, i.e. All, A28 and B17. Absorption experiments showed that the antibodies reactive with the neo-HLA antigens were not the same as those reactive with the specific HLA antigens. This alien expression of HLA-like structures was specific for the patients' prostatic tumor line, as EB-virus transformed B-lymphocytes of the patient expressed the genetically appropriate HLA antigens.
利用补体依赖细胞毒性试验并辅以吸收实验，对永久性人肿瘤细胞系PC - 93进行了HLA分型。与该患者的外周血淋巴细胞相比（PC - 93源自该患者的前列腺腺癌），观察到了显著差异。该肿瘤细胞系缺乏基因匹配的HLA - A1、A2和B15抗原的表达。PC - 93上似乎表达了与这些抗原交叉反应的HLA抗原，即A11、A28和B17。吸收实验表明，与新HLA抗原反应的抗体与与特异性HLA抗原反应的抗体不同。这种HLA样结构的异常表达是该患者前列腺肿瘤细胞系所特有的，因为该患者的EB病毒转化B淋巴细胞表达了基因匹配的HLA抗原。
