[Effect of trypsin inhibitor on the proteolysis of chicken ovalbumin in the rat gastrointestinal system].

The authors studied the effect of soybean trypsin inhibitor (SBTI) on the preservation of antigenic determinants of chicken ovalbumin (OVA) in different parts of the gastrointestinal tract of adult rats given both proteins at a time. Predetermined the activity of trypsin in the small intestinal contents of rats given protein mixtures containing SBTI for 20 and 40 minutes. Marked inhibition of trypsin activity was observed during both time intervals. Protein structures of OVA that maintained the initial antigenicity were assayed by the immunofluorescent technique. It was found that following 20 minutes the integrity of antigenic determinants of OVA during feeding with SBTI mixtures was significantly higher in both parts of the small intestine, Meanwhile after one hour there were significant differences in the indicators in the distal portion and in the small intestine on the whole. The authors discuss the possibility of the effect of SBTI introduced with a protein mixture on the penetration of "intact" or partially decomposed protein with preserved antigenic properties into the internal medium of the body.