小鼠和大鼠脱氧胞苷代谢的差异。
Differences in deoxycytidine metabolism in mouse and rat.
作者信息
Chan T S, Lakhchaura B D, Hsu T F
出版信息
Biochem J. 1983 Feb 15;210(2):367-71. doi: 10.1042/bj2100367.
Bone-marrow macrophages from both rat and mouse release deoxycytidine derived from phagocytosed nuclei. Mouse plasma contains no detectable deoxycytidine (less than 0.1 microM), whereas the concentration in rat plasma is 18 microM. Enzyme assays of tissue extracts show that both mouse and rat spleen contain high deoxycytidine kinase activity. Mouse organs, including kidney, liver and lung, also have deoxycytidine deaminase activity. In contrast, rat tissues have virtually no deoxycytidine deaminase activity. Lack of deaminase provides an explanation for the presence of deoxycytidine in rat plasma. Cytotoxicity assays show that cultured mouse lymphoid cells grown in undialysed rat serum are more resistant to cytotoxic effects of deoxyadenosine than are those cells grown in dialysed rat serum. The results suggest that a major difference in deoxycytidine metabolism between mouse and rat may account for discrepancies in the pharmacological response of the two animals to certain nucleoside compounds.
来自大鼠和小鼠的骨髓巨噬细胞会释放源自吞噬细胞核的脱氧胞苷。小鼠血浆中检测不到脱氧胞苷（低于0.1微摩尔/升），而大鼠血浆中的浓度为18微摩尔/升。组织提取物的酶分析表明，小鼠和大鼠的脾脏都具有高脱氧胞苷激酶活性。小鼠的器官，包括肾脏、肝脏和肺，也具有脱氧胞苷脱氨酶活性。相比之下，大鼠组织几乎没有脱氧胞苷脱氨酶活性。脱氨酶的缺乏解释了大鼠血浆中脱氧胞苷的存在。细胞毒性试验表明，在未透析的大鼠血清中培养的小鼠淋巴细胞比在透析的大鼠血清中培养的细胞对脱氧腺苷的细胞毒性作用更具抗性。结果表明，小鼠和大鼠在脱氧胞苷代谢上的主要差异可能解释了这两种动物对某些核苷化合物药理反应的差异。
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