Pitzurra M, Bistoni F, Pitzurra L, Marconi P
Bull World Health Organ. 1983;61(2):331-8.
Passive haemagglutination (HA) assays were performed using turkey red blood cells (TRBC-HA) on sera from normal healthy people, from normal people previously immunized against tetanus, and from tetanus patients receiving human antitetanus immunoglobulins. The TRBC-HA test was compared with haemagglutination assays using sheep red blood cells (SRBC-HA) and with the neutralization (NT) test, and was found to be more sensitive than the SRBC-HA test and showed good correlation with the NT test.While the SRBC-HA assay calls for adsorption of sheep red blood cell agglutinins from the sera to be tested, the use of turkey red blood cells does not require any such adsorption. In addition, the TRBC-HA assay can be performed in 40 minutes compared with 6 hours for the SRBC-HA assay. All these advantages make the TRBC-HA assay a more useful test for screening large numbers of sera in the evaluation of tetanus immunity of normal people and of patients with wounds when seen in the emergency room of hospitals.
采用火鸡红细胞(TRBC-HA)对正常健康人、既往接种过破伤风疫苗的正常人以及接受人抗破伤风免疫球蛋白治疗的破伤风患者的血清进行被动血凝试验(HA)。将TRBC-HA试验与采用绵羊红细胞的血凝试验(SRBC-HA)以及中和试验(NT)进行比较,发现其比SRBC-HA试验更敏感,且与NT试验具有良好的相关性。虽然SRBC-HA试验要求从待检测血清中吸附绵羊红细胞凝集素,但使用火鸡红细胞则无需进行此类吸附。此外,TRBC-HA试验可在40分钟内完成,而SRBC-HA试验则需要6小时。所有这些优点使得TRBC-HA试验在评估正常人和医院急诊室中伤口患者的破伤风免疫力时,成为一种更适用于筛查大量血清的有用检测方法。