Gullberg M, Larsson E L
Eur J Immunol. 1983 Jun;13(6):476-80. doi: 10.1002/eji.1830130609.
The effects of anti-Lyt-2 antibodies at different levels of lectin-induced cytotoxic T lymphocyte (CTL) responses have been analyzed. Induction of interleukin 2 (T cell growth factor) reactivity ("step 1") by three different lectins was profoundly inhibited by anti-Lyt-2 antibodies. Also lectin-dependent cytotoxicity of polyclonally induced CTL was significantly inhibited by anti-Lyt-2 antibodies, whereas the interjacent IL2-dependent proliferation of "step 1"-induced CTL precursors was unaffected by these antibodies. The anti-Lyt-2-mediated inhibition was selective and specific to the target cell determinants and limited to the events in CTL responses which require interactions with the lectins. These results parallel those obtained in antigen-induced responses and indicate that antigens and lectins induce CTL responses by interacting with the same type of membrane receptor complex containing Lyt-2 molecules and antigen-specific receptors. It follows that T cell mitogenic lectins do not appear to deliver triggering signals through "mitogen" receptors.
我们分析了抗Lyt-2抗体在不同水平的凝集素诱导细胞毒性T淋巴细胞（CTL）反应中的作用。三种不同的凝集素诱导白细胞介素2（T细胞生长因子）反应性（“步骤1”）受到抗Lyt-2抗体的显著抑制。此外，抗Lyt-2抗体也显著抑制了多克隆诱导的CTL的凝集素依赖性细胞毒性，而“步骤1”诱导的CTL前体的中间白细胞介素2依赖性增殖不受这些抗体的影响。抗Lyt-2介导的抑制作用具有选择性，对靶细胞决定簇具有特异性，并且仅限于CTL反应中需要与凝集素相互作用的事件。这些结果与在抗原诱导反应中获得的结果相似，表明抗原和凝集素通过与包含Lyt-2分子和抗原特异性受体的同一类型膜受体复合物相互作用来诱导CTL反应。由此可见，T细胞有丝分裂原凝集素似乎不会通过“有丝分裂原”受体传递触发信号。
