单克隆抗Lyt-2.2抗体可阻断H-2阴性靶细胞的凝集素依赖性细胞毒性。
Monoclonal anti-Lyt-2.2 antibody blocks lectin-dependent cellular cytotoxicity of H-2-negative target cells.
作者信息
Hünig T
出版信息
J Exp Med. 1984 Feb 1;159(2):551-8. doi: 10.1084/jem.159.2.551.
Abstract
The hypothesis that blocking of cytotoxic T lymphocyte (CTL)-mediated cytolysis with anti-Lyt-2 antibodies acts at the level of inhibiting the interaction of the Lyt-2-bearing structure with H-2 class I molecules was tested. In agreement with the findings of others, purified anti-Lyt-2.2 inhibited both antigen-specific lysis and lectin-dependent cellular cytotoxicity (LDCC). LDCC of H-2-positive and H-2-negative target cells was similarly inhibited by this antibody. As expected, this effect was specific for CTL expressing the Lyt-2.2 allele, in contrast to blocking with a rat monoclonal antibody to the murine LFA-1 antigen. The implications of this finding for the function of the Lyt-2 antigen in CTL-target cell interaction are discussed.
摘要
用抗Lyt-2抗体阻断细胞毒性T淋巴细胞（CTL）介导的细胞溶解作用于抑制携带Lyt-2结构与H-2 I类分子相互作用水平这一假说得到了验证。与其他人的研究结果一致，纯化的抗Lyt-2.2抑制了抗原特异性溶解和凝集素依赖性细胞毒性（LDCC）。该抗体同样抑制了H-2阳性和H-2阴性靶细胞的LDCC。正如预期的那样，与用抗小鼠LFA-1抗原的大鼠单克隆抗体进行阻断相反，这种效应对于表达Lyt-2.2等位基因的CTL是特异性的。讨论了这一发现对Lyt-2抗原在CTL-靶细胞相互作用中功能的影响。
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