Labrie F, Gagné B, Lefèvre G, Meunier H
Mol Cell Endocrinol. 1983 Jun;30(3):347-51. doi: 10.1016/0303-7207(83)90069-2.
Ovine corticotropin-releasing factor (CRF) stimulates adenylate cyclase activity in homogenate of the intermediate lobe of the bovine pituitary gland at an ED50 value of 150 nM. GTP increases the stimulatory effect induced by CRF as well as by the beta-adrenergic agonist isoproterenol on [32P]cyclic AMP formation in rat pars intermedia homogenate. In addition, GTP is required for inhibition by dopamine of the stimulatory action of both CRF and isoproterenol. The present data show that CRF stimulates adenylate cyclase activity in the intermediate lobe of the pituitary gland at least partly through a GTP-dependent mechanism. Moreover, dopamine can interfere with the action of CRF as well as that of isoproterenol, thus indicating that the neurohormone could be involved, in addition to beta-adrenergic agents, as stimulator of pars intermedia cell activity.
绵羊促肾上腺皮质激素释放因子（CRF）以150 nM的半数有效剂量（ED50）刺激牛垂体中间叶匀浆中的腺苷酸环化酶活性。GTP增强了CRF以及β-肾上腺素能激动剂异丙肾上腺素对大鼠垂体中间叶匀浆中[32P]环磷酸腺苷形成的刺激作用。此外，多巴胺抑制CRF和异丙肾上腺素的刺激作用需要GTP。目前的数据表明，CRF至少部分通过GTP依赖机制刺激垂体中间叶的腺苷酸环化酶活性。此外，多巴胺可干扰CRF以及异丙肾上腺素的作用，因此表明，除了β-肾上腺素能药物外，这种神经激素可能作为垂体中间叶细胞活性的刺激物参与其中。
