Sandberg G
Int Arch Allergy Appl Immunol. 1983;71(4):328-31.
Rosette-forming cells were counted in three thymocyte subpopulations separated by density gradient centrifugation with Percoll. A method for the electronic counting of rosettes is described which resulted in excellent agreement with results of visual counting. By both methods, significant differences in the rosette-forming ability of the studied subpopulations were detected. Thus, in population Ia, earlier shown to consist of large cells of low density and with high spontaneous proliferative activity, the rosette frequency was 54% as compared with 70% in the whole thymus population and 83% in population II, known to consist of small, immunologically incompetent cells. The third population studied (Ib), consisting of intermediate density cells, had 74% rosette-forming cells.
用Percoll通过密度梯度离心法分离出三个胸腺细胞亚群，对其中形成玫瑰花结的细胞进行计数。本文描述了一种玫瑰花结电子计数方法，该方法所得结果与目视计数结果高度一致。通过这两种方法，均检测到所研究亚群的玫瑰花结形成能力存在显著差异。因此，在Ia亚群中（先前显示该亚群由低密度的大细胞组成，具有高自发增殖活性），玫瑰花结频率为54%，而整个胸腺细胞群体中的玫瑰花结频率为70%，已知由小的、免疫无活性细胞组成的II亚群中的玫瑰花结频率为83%。所研究的第三个亚群（Ib）由中等密度细胞组成，其玫瑰花结形成细胞比例为74%。
