Sandberg G, Björkholm M
Clin Exp Immunol. 1985 Feb;59(2):499-504.
We investigated the possibility of using a standard electronic laboratory cell counter, of a type which permits manual settings of discriminator and sensitivity levels, for the quantitation of E rosette forming human blood lymphocytes. The electronic counting was performed on rosette specimens prepared from 10 healthy donors, nine lymphoma patients and eight patients with chronic lymphocytic leukaemia (CLL) of B cell type. The simple procedure which was earlier developed for the electronic counting of thymocyte rosettes in guinea-pigs was used and now supplemented with a method for rapid, graphical determination of rosette frequency. The low level of E rosettes in CLL patients was easily distinguished from the higher level seen in the other groups and there was a good agreement between electronic and visual determinations. In samples with a high rosette frequency, the cell counter initially gave 10-30% lower values than when recorded visually. This discrepancy was corrected, however, by a minor adjustment of discriminator level and the introduction of a correction procedure for the presence of double rosettes. We conclude that the electronic counter can well be used for the counting of human rosette forming blood lymphocytes and, due to a high precision and large capacity, this approach would prove to be useful when screening of a large number of samples is required.
我们研究了使用一种标准电子实验室血细胞计数器（该类型允许手动设置鉴别器和灵敏度水平）对形成E花环的人血淋巴细胞进行定量分析的可能性。电子计数是对来自10名健康供体、9名淋巴瘤患者和8名B细胞型慢性淋巴细胞白血病（CLL）患者制备的花环标本进行的。采用了先前为豚鼠胸腺细胞花环电子计数开发的简单程序，现在补充了一种快速、图形化测定花环频率的方法。CLL患者中E花环的低水平很容易与其他组中较高的水平区分开来，电子计数和视觉测定之间有很好的一致性。在花环频率高的样本中，血细胞计数器最初给出的值比视觉记录时低10% - 30%。然而，通过对鉴别器水平进行微小调整并引入针对双花环存在的校正程序，这种差异得到了校正。我们得出结论，电子计数器可以很好地用于计数形成花环的人血淋巴细胞，并且由于其高精度和大容量，当需要筛查大量样本时，这种方法将被证明是有用的。
