Cathers L E, Gould M N
Int J Radiat Biol Relat Stud Phys Chem Med. 1983 Jul;44(1):1-16. doi: 10.1080/09553008314550821.
We have developed and evaluated methods of culturing defined stromal and epithelial populations of normal human breast cells. These cell populations were used to generate radiation dose/survival curves. The epithelial cell population required specific hormones, growth factors, and conditioned media, as well as fibroblast feeder layers for clonal growth. Stromal cells grew well in a less complex medium. The stromal and parenchymal cell populations of the normal human breast were characterized by light and electron microscopy, immunohistochemical human fibronectin staining, gamma glutamyltranspeptidase histochemical staining, and cell sizing. Survival curves were generated using cells from four donors. The average D0 for epithelial cells was 122 cGy, with an average n value of 2.4. The average D0 and n values for stromal cells were 114 cGy and 2.0. The survival of human breast epithelial cells is compared to that of the cells of the rat mammary gland. The D0 values of both species are essentially the same, while the n value for human epithelial cells is lower. This difference in the n value may be a species specific response to radiation, or may merely reflect a difference in the two assay systems used to generate the survival curves.
我们已经开发并评估了培养正常人乳腺细胞特定基质和上皮细胞群体的方法。这些细胞群体被用于生成辐射剂量/存活曲线。上皮细胞群体需要特定的激素、生长因子和条件培养基，以及用于克隆生长的成纤维细胞饲养层。基质细胞在不太复杂的培养基中生长良好。通过光学显微镜和电子显微镜、免疫组织化学人纤连蛋白染色、γ-谷氨酰转肽酶组织化学染色以及细胞大小测定对正常人乳腺的基质细胞和实质细胞群体进行了表征。使用来自四名供体的细胞生成了存活曲线。上皮细胞的平均D0为122 cGy，平均n值为2.4。基质细胞的平均D0和n值分别为114 cGy和2.0。将人乳腺上皮细胞的存活率与大鼠乳腺细胞的存活率进行了比较。两种物种的D0值基本相同，而人上皮细胞的n值较低。n值的这种差异可能是对辐射的物种特异性反应，或者可能仅仅反映了用于生成存活曲线的两种检测系统的差异。
