Vullings H G, Diederen J H
Histochemistry. 1983;77(3):405-14. doi: 10.1007/BF00490901.
The secretory material within the cells of the subcommissural organ (SCO) of Rana temporaria has been studied histochemically by application of a classical staining method and immunocytochemically by use of an antiserum (gift of Prof. Sterba, Leipzig) raised against bovine liquor fibre (LF) material, the LF being the secretory product of the SCO. The immunocytochemical method appears to be more specific and more sensitive than the histochemical staining method. The LF can also be visualized immunocytochemically; the fibre reacting equally positively from the SCO until the spinal cord's end. A significant positive linear correlation exists both between the amounts of histochemically and of immunocytochemically stained material and between their concentrations. The suitability of the immunocytochemical staining method for quantification of the secretory material within the cells of the SCO is discussed.
通过应用经典染色方法，对林蛙视交叉下器官（SCO）细胞内的分泌物质进行了组织化学研究，并使用针对牛脑液纤维（LF）物质产生的抗血清（由莱比锡的斯特尔巴教授馈赠）进行了免疫细胞化学研究，其中LF是SCO的分泌产物。免疫细胞化学方法似乎比组织化学染色方法更具特异性和敏感性。LF也可以通过免疫细胞化学进行可视化；从SCO到脊髓末端，纤维的反应均呈阳性。组织化学染色物质的量与免疫细胞化学染色物质的量之间以及它们的浓度之间均存在显著的正线性相关性。讨论了免疫细胞化学染色方法用于定量SCO细胞内分泌物质的适用性。
