Lim C, Lovell R T
J Nutr. 1978 Jul;108(7):1137-46. doi: 10.1093/jn/108.7.1137.
Channel catfish fingerlings fed purified diets devoid of vitamin C showed reduced growth rate, deformed spinal columns, external and internal hemorrhages, erosion of fins, dark skin color and reduced bone collagen content after 8 to 12 weeks, whereas fish fed a diet containing 30 mg/kg of vitamin C had none of these anomalies after 22 weeks. A dietary level of 30 mg of vitamin C per kg was insufficient to prevent distortion of gill filament cartilage, although 60 mg of vitamin C per kg was sufficient. Vertebral collagen percentages of 25 or below and liver ascorbic acid levels of 30 microgram/g or below appeared to be indicative of vitamin C deficiency in channel catfish fingerlings. Epidermis and dermis were almost completely healed and extensive collagen fiber formation had commenced in the somatic muscle, in experimentally inflicted wounds after 10 days in fish fed the vitamin C-free diet. Skin and muscle at the wound site were almost regenerated to normal after 10 days in fish fed 60 mg of vitamin C per kg of diet.
饲喂不含维生素C的纯化日粮8至12周后，斑点叉尾鮰鱼种生长速率降低，出现脊柱畸形、内外出血、鳍糜烂、皮肤颜色变深以及骨胶原蛋白含量降低的情况；而饲喂含30毫克/千克维生素C日粮的鱼在22周后未出现这些异常情况。每千克日粮中30毫克的维生素C含量不足以防止鳃丝软骨变形，不过每千克60毫克的维生素C含量则足够。椎骨胶原蛋白百分比在25或以下以及肝脏抗坏血酸水平在30微克/克或以下似乎表明斑点叉尾鮰鱼种存在维生素C缺乏的情况。在饲喂不含维生素C日粮的鱼中，实验造成的伤口在10天后，表皮和真皮几乎完全愈合，体肌中开始大量形成胶原纤维。在饲喂每千克日粮含60毫克维生素C的鱼中，伤口部位的皮肤和肌肉在10天后几乎恢复正常。
