Xu Cong-Mei, Yu Hai-Rui, Li Ling-Yao, Li Min, Qiu Xiang-Yi, Fan Xiao-Qian, Fan Yan-Lin, Shan Ling-Ling
Key Laboratory of Biochemistry and Molecular Biology in Universities of Shandong (Weifang University), Weifang Key Laboratory of Coho Salmon Culturing Facility Engineering, Institute of Modern Facility Fisheries, College of Biology and Oceanography, Weifang University, Weifang 261061, China.
Shandong Collaborative Innovation Center of Coho Salmon Health Culture Engineering Technology, Shandong Conqueren Marine Technology Co., Ltd., Weifang 261108, China.
Aquac Nutr. 2022 Dec 26;2022:6866578. doi: 10.1155/2022/6866578. eCollection 2022.
Vitamin C (VC) plays an essential role in fish physiological function and normal growth. However, its effects and requirement of coho salmon (Walbaum, 1792) are still unknown. Based on the influences on growth, serum biochemical parameters, and antioxidative ability, an assessment of dietary VC requirement for coho salmon postsmolts (183.19 ± 1.91 g) was conducted with a ten-week feeding trial. Seven isonitrogenous (45.66% protein) and isolipidic (10.76% lipid) diets were formulated to include graded VC concentrations of 1.8, 10.9, 50.8, 100.5, 197.3, 293.8, and 586.7 mg/kg, respectively. Results showed that VC markedly improved the growth performance indexes and liver VC concentration, enhanced the hepatic and serum antioxidant activities, and increased the contents of serum alkaline phosphatase (AKP) activity, low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), and total cholesterol (TC) whereas decreased the serum aspartate aminotransferase (AST), alanine aminotransferase (ALT) activities, and triglyceride (TG) level. Polynomial analysis showed that the optimal VC levels in the diet of coho salmon postsmolts were 188.10, 190.68, 224.68, 132.83, 156.57, 170.12, 171.00, 185.50, 142.77, and 93.08 mg/kg on the basis of specific growth rate (SGR), feed conversion ratio (FCR), liver VC concentration, catalase (CAT), hepatic superoxide dismutase (SOD) activities, malondialdehyde (MDA) content, and serum total antioxidative capacity (T-AOC), AKP, AST, and ALT activities, respectively. The dietary VC requirement was in the range of 93.08-224.68 mg/kg for optimum growth performance, serum enzyme activities, and antioxidant capacity of coho salmon postsmolts.
维生素C（VC）在鱼类生理功能和正常生长中起着至关重要的作用。然而，其对银大麻哈鱼（Walbaum，1792）的影响和需求量仍不清楚。基于对生长、血清生化参数和抗氧化能力的影响，通过为期十周的饲养试验，对银大麻哈鱼幼鲑（183.19±1.91克）的饲料VC需求量进行了评估。配制了七种等氮（蛋白质含量45.66%）和等脂（脂肪含量10.76%）的饲料，其VC浓度分别为1.8、10.9、50.8、100.5、197.3、293.8和586.7毫克/千克。结果表明，VC显著提高了生长性能指标和肝脏VC浓度，增强了肝脏和血清的抗氧化活性，增加了血清碱性磷酸酶（AKP）活性、低密度脂蛋白胆固醇（LDL-C）、高密度脂蛋白胆固醇（HDL-C）和总胆固醇（TC）的含量，而降低了血清天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）活性和甘油三酯（TG）水平。多项式分析表明，基于特定生长率（SGR）、饲料转化率（FCR）、肝脏VC浓度、过氧化氢酶（CAT）、肝脏超氧化物歧化酶（SOD）活性、丙二醛（MDA）含量以及血清总抗氧化能力（T-AOC）、AKP、AST和ALT活性，银大麻哈鱼幼鲑饲料中最佳VC水平分别为188.10、190.68、224.68、132.83、156.57、170.12、171.00、185.50、142.77和93.08毫克/千克。为使银大麻哈鱼幼鲑获得最佳生长性能、血清酶活性和抗氧化能力，饲料中VC需求量在93.08 - 224.68毫克/千克范围内。
