Brandslund I, Teisner B, Hole P, Grudzinskas J G, Svehag S E
Acta Pathol Microbiol Immunol Scand C. 1983 Feb;91(1):51-7.
The activation of complement (C) factors in a patient exhibiting an anaphylactoid shock 10 min after the initiation of transurethral coagulation of vesical papillomas was studied. Streptococcus faecalis was cultured from he urine before and after the shock and from the blood on two occasions within 2 days after the shock. The shock was associated with a marked but transient complement activation as judged from serial determinations of C3d and C3c by doubledecker rocket immuno electrophoresis and native C3 and C3c by crossed immuno electrophoresis. Conventional total C3 quantitation by rocket immuno electrophoresis did not reveal the activation. A decrease of native C4 to 20% of normal values and the appearance of C4 split products indicated classical pathway activation. Factor B conversion showed the alternative pathway to be involved as well. Total haemolytic complement activity was temporarily reduced, increasing without reaching normal values within 20 hours. The results indicated that the classical pathway was transiently activated, pointing to an acute antigen-antibody reaction, probably caused by the entrance of bacteria and bacterial products into the circulation of an immunized host.
对一名在膀胱乳头状瘤经尿道凝固术开始后10分钟出现类过敏性休克的患者体内补体（C）因子的激活情况进行了研究。在休克前后的尿液以及休克后2天内两次从血液中培养出粪肠球菌。根据用双层火箭免疫电泳连续测定C3d和C3c以及用交叉免疫电泳测定天然C3和C3c判断，休克与显著但短暂的补体激活相关。用火箭免疫电泳进行的常规总C3定量未显示激活情况。天然C4降至正常值的20%以及C4裂解产物的出现表明经典途径被激活。B因子转化表明替代途径也参与其中。总溶血补体活性暂时降低，在20小时内虽有升高但未达到正常值。结果表明经典途径被短暂激活，提示存在急性抗原 - 抗体反应，可能是由细菌和细菌产物进入免疫宿主的循环系统所致。
