Yano A, Yui K, Aosai F, Kojima S, Kawana T, Ovary Z
Int Arch Allergy Appl Immunol. 1983;72(2):150-7.
The immune response of patients with trichomoniasis vaginitis to Trichomonas vaginalis was examined by using passive hemagglutination assay and skin reactions. Trichomonas-specific immune responses could hardly be detected in the patients by these assay methods. In order to develop a simple and reliable assay for demonstration of T. vaginalis-specific immune responses in the patients, proliferation responses of peripheral mononuclear cells from the patients were examined. Lymphocytes obtained from the patients were shown to incorporate 3H-methyl-thymidine when stimulated with T. vaginalis antigen in vitro. Furthermore, immunochemical analysis of T. vaginalis antigen has been done by Sephadex chromatography and sodium dodecyl sulfate gel electrophoresis. The data revealed that the murine and human IgM antibodies specific to T. vaginalis recognize various antigens with a wide range of molecular weights (between 13,000 and 100,000 daltons), while human and murine IgG antibodies recognize molecules with a much narrower range of molecular weights (50,000-100,000 daltons).
采用被动血凝试验和皮肤反应检测了滴虫性阴道炎患者对阴道毛滴虫的免疫反应。通过这些检测方法，在患者中几乎检测不到阴道毛滴虫特异性免疫反应。为了开发一种简单可靠的检测方法来证明患者体内阴道毛滴虫特异性免疫反应，检测了患者外周血单核细胞的增殖反应。结果显示，从患者获取的淋巴细胞在体外受到阴道毛滴虫抗原刺激时会掺入3H-甲基胸腺嘧啶核苷。此外，已通过葡聚糖凝胶色谱法和十二烷基硫酸钠凝胶电泳对阴道毛滴虫抗原进行了免疫化学分析。数据显示，针对阴道毛滴虫的鼠源和人源IgM抗体识别各种分子量范围较宽（13,000至100,000道尔顿之间）的抗原，而人和鼠源IgG抗体识别分子量范围窄得多（50,000 - 100,000道尔顿）的分子。
