大鼠下丘脑促肾上腺皮质激素释放因子的特性研究
Characterization of rat hypothalamic corticotropin-releasing factor.
作者信息
Rivier J, Spiess J, Vale W
出版信息
Proc Natl Acad Sci U S A. 1983 Aug;80(15):4851-5. doi: 10.1073/pnas.80.15.4851.
Abstract
A polypeptide was purified from rat hypothalamic extracts on the basis of its high intrinsic activity to release corticotropin (ACTH) from cultured rat anterior pituitary cells and its immunoactivity in a radioimmunoassay directed against the NH2 terminus (residues 4-20) of ovine hypothalamic corticotropin-releasing factor (CRF). Based on Edman degradation, peptide mapping, and amino acid analysis, the primary structure of this rat CRF was established to be: H-Ser-Glu-Glu-Pro-Pro-Ile-Ser-Leu-Asp-Leu-Thr-Phe-His-Leu-Leu-Arg-Glu-Val-Leu-Glu-Met-Ala-Arg-Ala-Glu-Gln-Leu-Ala-Gln-Gln-Ala-His-Ser-Asn-Arg-Lys-Leu-Met-Glu-Ile-Ile-NH2. The hypophysiotropic potency of synthetic rat CRF did not deviate significantly from the potencies of the isolated native peptide or of synthetic ovine CRF. The close structural relationship between rat and ovine hypothalamic CRF is indicated by an 83% sequence homology.
摘要
一种多肽是从大鼠下丘脑提取物中纯化出来的，其依据是它能从培养的大鼠垂体前叶细胞中高效释放促肾上腺皮质激素（ACTH），并且在针对羊下丘脑促肾上腺皮质激素释放因子（CRF）的NH2末端（第4至20位氨基酸残基）的放射免疫分析中具有免疫活性。基于埃德曼降解法、肽图谱分析和氨基酸分析，确定这种大鼠CRF的一级结构为：H-Ser-Glu-Glu-Pro-Pro-Ile-Ser-Leu-Asp-Leu-Thr-Phe-His-Leu-Leu-Arg-Glu-Val-Leu-Glu-Met-Ala-Arg-Ala-Glu-Gln-Leu-Ala-Gln-Gln-Ala-His-Ser-Asn-Arg-Lys-Leu-Met-Glu-Ile-Ile-NH2。合成的大鼠CRF对垂体的作用效能与分离得到的天然肽或合成的羊CRF的效能相比，没有显著差异。大鼠和羊下丘脑CRF之间紧密的结构关系通过83%的序列同源性得以体现。
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