Brazeau P, Böhlen P, Esch F, Ling N, Wehrenberg W B, Guillemin R
Biochem Biophys Res Commun. 1984 Dec 14;125(2):606-14. doi: 10.1016/0006-291x(84)90582-5.
Peptides with high intrinsic growth hormone releasing activity (growth hormone-releasing factor, GRF) were isolated from 2100 ovine and 2600 caprine (goat) hypothalami by means of acid extraction, immunoaffinity chromatography, gel filtration and reverse phase HPLC. Structural characterization of the 44 amino acid ovine peptide by gas-liquid phase sequencing and peptide mapping established its primary structure as Tyr-Ala-Asp-Ala-Ile-Phe-Thr-Asn-Ser -Tyr-Arg-Lys-Ile-Leu-Gly-Gln-Leu-Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp-Ile-Met -Asn -Arg-Gln-Gln-GLy-Glu-Arg-Asn-Gln-Glu-Gln-Gly-Ala-Lys-Val-Arg-Leu-NH2. Caprine GRF was found to possess the same sequence except for the replacement of the isoleucine residue in position 13 with valine and thus is identical to bovine GRF.
通过酸提取、免疫亲和色谱、凝胶过滤和反相高效液相色谱法，从2100个绵羊下丘脑和2600个山羊下丘脑分离出具有高内在生长激素释放活性的肽（生长激素释放因子，GRF）。通过气液相测序和肽图谱对44个氨基酸的绵羊肽进行结构表征，确定其一级结构为Tyr-Ala-Asp-Ala-Ile-Phe-Thr-Asn-Ser-Tyr-Arg-Lys-Ile-Leu-Gly-Gln-Leu-Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp-Ile-Met-Asn-Arg-Gln-Gln-GLy-Glu-Arg-Asn-Gln-Glu-Gln-Gly-Ala-Lys-Val-Arg-Leu-NH2。发现山羊GRF除第13位异亮氨酸残基被缬氨酸取代外，具有相同序列，因此与牛GRF相同。
