Fontana A, McAdam K P, Kristensen F, Weber E
Eur J Immunol. 1983 Aug;13(8):685-9. doi: 10.1002/eji.1830130814.
It has been previously reported that lipopolysaccharide-stimulated murine astrocytes produce a factor which enhances the proliferative response of thymocytes to lectins. The present report demonstrates that a rat astrocytoma cell (C6 cells)-derived factor appears to be similar to macrophage-derived interleukin 1 (IL 1) in its biological activities and biochemical characteristics. Upon injection into mice, supernatants of C6 cells induce the production of serum amyloid A. The C6 cell-derived factors enhance the response of thymocytes to phytohemagglutinin and the growth of fibroblasts whereas no effect on the growth of neuroblasts and of a strictly interleukin 2 (IL 2)-dependent T cell line was observed. On an AcA 54 column the C6-derived factors acting on thymocytes and fibroblasts coeluted as a single peak of Mr = 13 500 to 18 000; the semipurified factor was found to enhance the lymphocytes' production of IL 2. These observations demonstrate that cells not belonging to the mononuclear phagocyte lineage are able to produce factors identical or closely related to IL 1.
先前已有报道称，脂多糖刺激的小鼠星形胶质细胞会产生一种因子，该因子可增强胸腺细胞对凝集素的增殖反应。本报告表明，大鼠星形细胞瘤细胞（C6细胞）衍生的因子在生物学活性和生化特性上似乎与巨噬细胞衍生的白细胞介素1（IL-1）相似。将C6细胞的上清液注射到小鼠体内后，可诱导血清淀粉样蛋白A的产生。C6细胞衍生的因子可增强胸腺细胞对植物血凝素的反应以及成纤维细胞的生长，而对神经母细胞和严格依赖白细胞介素2（IL-2）的T细胞系的生长没有影响。在AcA 54柱上，作用于胸腺细胞和成纤维细胞的C6衍生因子以Mr = 13500至18000的单峰形式共洗脱；发现半纯化因子可增强淋巴细胞产生IL-2的能力。这些观察结果表明，不属于单核吞噬细胞系的细胞能够产生与IL-1相同或密切相关的因子。
