一种由用腺病毒DNA转染的小鼠骨髓细胞衍生而来的集落刺激因子产生细胞系。
A colony-stimulating-factor-producing cell line derived from mouse bone marrow cells transfected with adenovirus DNA.
作者信息
Fujita J, Yoshida O, Miyanomae T, Mori K J
出版信息
Gan. 1983 Jun;74(3):334-7.
PMID:6603995
Abstract
A unique fibroblastoid cell line, BMA1, has been established from bone marrow cells of a DDY mouse transfected with adenovirus 5 DNA. BMA1 cells expressed adenoviral early antigens and BMA1-conditioned medium contained mouse granulocyte/macrophage colony-stimulating factor(s). The DNA transfection technique may be useful in establishing cell lines from bone marrow and analyzing the regulation of hemopoiesis.
摘要
已从用腺病毒5 DNA转染的DDY小鼠的骨髓细胞中建立了一种独特的成纤维细胞样细胞系BMA1。BMA1细胞表达腺病毒早期抗原，且BMA1条件培养基含有小鼠粒细胞/巨噬细胞集落刺激因子。DNA转染技术可能有助于从骨髓建立细胞系并分析造血的调控。
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