Lanotte M, Metcalf D, Dexter T M
J Cell Physiol. 1982 Jul;112(1):123-7. doi: 10.1002/jcp.1041120118.
Three preadipocyte cell lines that have been independently derived from bone marrow stroma (Lanotte et al, 1982) have been tested for their capacity to produce granulocyte, macrophage, and erythroid colony-stimulating factors (CSFs). All elaborated colony-stimulating material that was active upon adult mouse marrow granulocyte/macrophage colony-forming cells (M-CFC) but not foetal liver GM-CFC. The major activity was characterised as a monocyte-macrophage colony-stimulating factor (M-CSF), and the pattern of colony stimulation was similar to that seen after addition of highly purified L-cell CSF. Furthermore, the stimulating activity was specifically neutralised by rabbit anti-L cell CSF antibodies. No evidence was found for stimulation of multipotential or erythroid colony-forming cells, only few granulocytic colonies were detected, and the stimulating activity had no mouse strain restriction. All cell lines produced large quantities of M-CSF; however, the production was found to be modulated during the adipogenesis process. A peak in M-CSF production corresponded to the period of growth arrest after confluence of the stromal cells was reached and when adipocyte maturation was at an early stage. A marked depression in M-CSF secretion was associated with the final steps of adipocyte maturation.
三种分别独立源自骨髓基质的前脂肪细胞系（Lanotte等人，1982年）已被检测其产生粒细胞、巨噬细胞和红细胞集落刺激因子（CSF）的能力。所有细胞系都产生了对成年小鼠骨髓粒细胞/巨噬细胞集落形成细胞（M-CFC）有活性，但对胎肝GM-CFC无活性的集落刺激物质。主要活性被鉴定为单核细胞-巨噬细胞集落刺激因子（M-CSF），其集落刺激模式与添加高度纯化的L细胞CSF后所见的模式相似。此外，刺激活性被兔抗L细胞CSF抗体特异性中和。未发现对多能或红细胞集落形成细胞有刺激作用的证据，仅检测到少数粒细胞集落，且刺激活性无小鼠品系限制。所有细胞系都产生大量的M-CSF；然而，发现其产生在脂肪生成过程中受到调节。M-CSF产生的峰值对应于基质细胞汇合后生长停滞且脂肪细胞成熟处于早期阶段的时期。M-CSF分泌的显著降低与脂肪细胞成熟的最后步骤相关。
