Iványi D, Démant P
J Immunol. 1983 Sep;131(3):1080-4.
Analysis of the antigenic heterogeneity of Db region products by the technique of antibody-induced redistribution of cell surface antigens (capping) revealed the existence of two types of H-2 molecules reactive with the monoclonal anti-Db antibody, B22-249R 1 (H-2.m2). The relationship of the two H-2.2-positive molecules described here is similar to that of H-2D and H-2M detected in the products of the Dd region; they differ in the repertoire of the H-2 public specificities, although they share the private specificity. We designate them, in accordance to recently proposed nomenclature, H-2D1b and H-2D2b. Both types of molecule have been detected on T lymphocytes of C57BL/6 (H-2b), C3H.B10(H-2b), and B10.A(2R) (H-2h2, KkDb) strains. In mutant strain B6.C-H-2bm13, however, only one type of H-2.2-positive molecule, H-2D1b, could be detected. This finding resembles the situation in the d haplotype, in which mutant strain B10.D2 (M504) (H-2dm1) had only one molecule (H-2Dd), of the two H-2.4-positive molecules H-2Dd and H-2Md that could be detected. A third Db region molecule, H-2Lb, does not carry the Db private specificity H-2.2, and it is detectable by some of the antibodies against the H-2.28 family of specificities; it is distinct from the Qa-2 molecule. The H-2Lb molecule was detected in all strains tested in this experiment, including the bm13 mutant.
通过抗体诱导细胞表面抗原重分布（封帽）技术分析Db区域产物的抗原异质性，发现存在两种与单克隆抗-Db抗体B22-249R 1（H-2.m2）反应的H-2分子。此处描述的两种H-2.2阳性分子的关系类似于在Dd区域产物中检测到的H-2D和H-2M的关系；它们在H-2公共特异性的库中有所不同，尽管它们共享私有特异性。根据最近提出的命名法，我们将它们命名为H-2D1b和H-2D2b。在C57BL/6（H-2b）、C3H.B10（H-2b）和B10.A(2R)（H-2h2，KkDb）品系的T淋巴细胞上均检测到了这两种分子。然而，在突变品系B6.C-H-2bm13中，只能检测到一种H-2.2阳性分子，即H-2D1b。这一发现类似于d单倍型中的情况，在d单倍型中，突变品系B10.D2（M504）（H-2dm1）在可检测到的两种H-2.4阳性分子H-2Dd和H-2Md中只有一种分子（H-2Dd）。第三个Db区域分子H-2Lb不携带Db私有特异性H-2.2，并且可以被一些针对H-2.28特异性家族的抗体检测到；它与Qa-2分子不同。在本实验测试的所有品系中均检测到了H-2Lb分子，包括bm13突变体。
