Rosseel M T, Bogaert M G, Claeys M
J Pharm Sci. 1978 Jun;67(6):802-5. doi: 10.1002/jps.2600670619.
A GLC method for the simultaneous quantitative determination of the cis- and trans-isomers of doxepin and desmethyldoxepin in human plasma was developed. The method involves the use of a capillary column for efficient separation of the four compounds and the internal standards, amitriptyline and nortriptyline. A high sensitivity is obtained with a nitrogen detector, enabling quantitation of the compounds in plasma of humans treated chronically with doxepin. Confirmation of the identity of the cis- and trans-isomers of doxepin and desmethyldoxepin in biological samples was carried out by selected ion monitoring.
建立了一种同时定量测定人血浆中多塞平及其去甲基代谢物顺式和反式异构体的气相色谱法。该方法采用毛细管柱高效分离这四种化合物及内标物阿米替林和去甲替林。使用氮检测器可获得高灵敏度，能够对长期接受多塞平治疗的人的血浆中的化合物进行定量。通过选择离子监测对生物样品中多塞平及其去甲基代谢物的顺式和反式异构体进行了定性确证。
