Riis-Vestergaard J
J Biochem Biophys Methods. 1983 Jul;7(4):285-91. doi: 10.1016/0165-022x(83)90053-2.
The diffusion coefficients of radioactively labelled substances in cytoplasm or other fluids are determined in vitro. The fluid containing the labelled substance is filled into a cylinder with one open end, through which the labelled substance diffuses out into a stirred outer medium. The diffusion coefficient is calculated by a one-dimensional diffusion equation from the rate of loss from the cylinder, and the length of the cylinder. The diffusion coefficients of tritiated water in several fluids have been determined. The results are in good agreement with those obtained by other methods.
在体外测定放射性标记物质在细胞质或其他液体中的扩散系数。将含有标记物质的液体装入一端开口的圆柱体内，标记物质通过该开口扩散到搅拌的外部介质中。根据圆柱体内物质损失的速率以及圆柱体的长度，利用一维扩散方程计算扩散系数。已测定了氚水在几种液体中的扩散系数。结果与通过其他方法获得的结果高度一致。
