Nakano N I, Oshio T, Fujimoto Y, Amiya T
J Pharm Sci. 1978 Jul;67(7):1005-8. doi: 10.1002/jps.2600670737.
The affinity chromatographic technique was used to study the interaction of bovine serum albumin and salicylic acid at 3.3 +/- 1.1 degrees. Beaded agarose gel, on which the albumin was immobilized by covalent linkage, was packed in a column as an affinity adsorbent. Frontal analysis was performed on this column to evaluate the binding parameters for the interaction. The effect of albumin immobilization on drug binding was investigated by comparing the binding parameters of two affinity adsorbents, directly coupled albumin and albumin coupled through a spacer arm. The latter mode of attachment gave binding characteristics comparable to those of the soluble albumin. The method is simple and precise. The affinity adsorbent can be used repeatedly for many months for various drugs, including those that do not diffuse through dialysis membranes.
采用亲和色谱技术研究了牛血清白蛋白与水杨酸在3.3±1.1摄氏度下的相互作用。通过共价连接将白蛋白固定在珠状琼脂糖凝胶上，将其填充到柱中作为亲和吸附剂。对该柱进行前沿分析以评估相互作用的结合参数。通过比较两种亲和吸附剂（直接偶联的白蛋白和通过间隔臂偶联的白蛋白）的结合参数，研究了白蛋白固定化对药物结合的影响。后一种连接方式产生的结合特性与可溶性白蛋白相当。该方法简单且精确。亲和吸附剂可反复使用数月，用于多种药物，包括那些不能透过透析膜扩散的药物。
