Nakano N I, Shimamori Y, Yamaguchi S
J Chromatogr. 1980 Feb 1;188(2):347-56. doi: 10.1016/s0021-9673(00)81257-1.
A continuous frontal analysis chromatographic method was developed for studying the simultaneous binding of two drugs or ligands with an immobilized macromolecule. The usefulness of this method was demonstrated in the interactions of sulphamethizole and salicylic acid with human serum albumin (HSA). The mutual inhibitory effect on the binding of one drug of the presence of the other was directly shown to be due to displacement of the bound drug from HSA by the other. On the basis of a double-reciprocal plot analysis, these two drugs are interpreted as competing for the same primary binding sites.
开发了一种连续前沿分析色谱方法，用于研究两种药物或配体与固定化大分子的同时结合。该方法在磺胺甲噻二唑和水杨酸与人血清白蛋白（HSA）的相互作用中得到了验证。直接表明，一种药物的存在对另一种药物结合的相互抑制作用是由于另一种药物将结合在HSA上的药物置换所致。基于双倒数图分析，这两种药物被解释为竞争相同的主要结合位点。
