采用高压液相色谱法快速测定人血浆和尿液中的阿替洛尔。

Rapid determination of atenolol in human plasma and urine by high-pressure liquid chromatography.

作者信息

Weddle O H, Amick E N, Mason W D

出版信息

J Pharm Sci. 1978 Jul;67(7):1033-5. doi: 10.1002/jps.2600670748.

Abstract

A rapid, specific, high-pressure liquid chromatographic determination of atenolol in plasma and urine was developed. This method employs the high sensitivity of fluorescence detection together with selective extraction and reversed-phase chromatography to measure concentrations as low as 20 ng of drug/ml of plasma with a coefficient of variation of 3.91%. The assay is specific enough to be valid in the presence of plasma and urine substances. The detection limit (i.e., three times baseline noise) is 3 ng/ml.

摘要

建立了一种快速、特异的血浆和尿液中阿替洛尔的高压液相色谱测定法。该方法利用荧光检测的高灵敏度，结合选择性萃取和反相色谱法，可测定低至20 ng药物/毫升血浆的浓度，变异系数为3.91%。该测定法特异性高，在血浆和尿液物质存在的情况下仍有效。检测限（即三倍基线噪音）为3 ng/ml。