注射了偶氮胂III或安替比拉宗III的完整青蛙肌肉纤维的各向同性钙信号比较。
Comparison of isotropic calcium signals from intact frog muscle fibers injected with Arsenazo III or Antipyrylazo III.
作者信息
Baylor S M, Quinta-Ferreira M E, Hui C S
出版信息
Biophys J. 1983 Oct;44(1):107-12. doi: 10.1016/S0006-3495(83)84282-9.
Abstract
Intact single skeletal muscle fibers were micro-injected with either of the metallochromic indicator dyes Arsenazo III or Antipyrylazo III, and dye-related Ca2+ signals from each were measured during a twitch. In comparison with the Arsenazo III Ca2+ signal, the signal from Antipyrylazo III had three favorable features: (a) it was temporally faster, (b) its spectral dependence agreed with a cuvette calibration, and (c) its kinetic behavior was consistent with a single Ca2+ -dye stoichiometry. It is therefore suggested that the Antipyrylazo III Ca2+ signal is a more accurate monitor of the time course of the underlying myoplasmic free Ca2+ transient and one that may be more reliably calibrated.
摘要
完整的单个骨骼肌纤维被微量注射金属显色指示剂染料偶氮胂III或安替比拉佐III中的一种，并在一次单收缩过程中测量每种染料相关的Ca2+信号。与偶氮胂III的Ca2+信号相比，安替比拉佐III的信号有三个有利特征：(a) 它在时间上更快；(b) 其光谱依赖性与比色皿校准一致；(c) 其动力学行为与单一Ca2+ -染料化学计量一致。因此，有人认为安替比拉佐III的Ca2+信号是对潜在肌质游离Ca2+瞬变时间进程更准确的监测器，并且可能更可靠地进行校准。
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