从外周神经培养物中纯化大鼠雪旺细胞：一种使用抗免疫球蛋白抗体包被表面的免疫选择方法。
Purification of rat Schwann cells from cultures of peripheral nerve: an immunoselective method using surfaces coated with anti-immunoglobulin antibodies.
作者信息
Assouline J G, Bosch E P, Lim R
出版信息
Brain Res. 1983 Oct 31;277(2):389-92. doi: 10.1016/0006-8993(83)90953-8.
PMID:6605783
Abstract
We report a method for deriving purified rat Schwann cells by immunoselective removal of fibroblasts. Contaminating fibroblasts labeled with antibody against specific surface marker Thy 1.1 are bound on plastic surfaces coated with a second antibody. The efficacy of the method is demonstrated by flow cytometry and by specific Schwann cell Ran-1 immunofluorescence.
摘要
我们报告了一种通过免疫选择性去除成纤维细胞来获得纯化大鼠雪旺细胞的方法。用抗特异性表面标志物Thy 1.1抗体标记的污染成纤维细胞与包被有二抗的塑料表面结合。通过流式细胞术和雪旺细胞特异性Ran-1免疫荧光证明了该方法的有效性。
Zotero 插件