Farrar G H, Glöckner W M, Uhlenbruck G
Klin Wochenschr. 1983 Oct 3;61(19):941-5. doi: 10.1007/BF01550266.
The carbohydrate composition of a purified plasma membrane fraction derived from the peripheral blood lymphocytes of a patient with a pro-lymphocytic leukaemia has been investigated and compared to the expression of lectin receptors at the membrane surface of the intact cell. The presence of galactose, mannose, fucose, glucose, sialic acid, N-acetylgalactosamine, N-acetylglucosamine and sialic acid substituted O-glycosidic linked beta-D-galactopyranosyl (1 leads to 3) N-acetyl-D-galactosamine was confirmed on membrane glycoconjugates by gas chromatography. A lectin induced agglutinin assay using intact leukaemia cells demonstrated the accessibility of different lectin receptors at the native membrane surface, while the peanut agglutinin and the Helix pomatia receptor was only accessible after neuraminidase treatment.
对一名原淋巴细胞白血病患者外周血淋巴细胞来源的纯化质膜组分的碳水化合物组成进行了研究，并与完整细胞表面膜上凝集素受体的表达进行了比较。通过气相色谱法在膜糖缀合物上证实了半乳糖、甘露糖、岩藻糖、葡萄糖、唾液酸、N-乙酰半乳糖胺、N-乙酰葡糖胺以及唾液酸取代的O-糖苷键连接的β-D-吡喃半乳糖基（1→3）N-乙酰-D-半乳糖胺的存在。使用完整白血病细胞的凝集素诱导凝集试验证明了天然膜表面不同凝集素受体的可及性，而花生凝集素和苹果蜗牛受体仅在神经氨酸酶处理后才可及。
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