de Dios I, Manso M, León V, López-Borrasca A
Rev Esp Fisiol. 1984 Sep;40(3):265-70.
Plasma membranes composition and structure of B, T, T gamma and T mu lymphocytes from peripheral blood have been studied using four 125I-labelled lectins. The results showed that B lymphocytes have a greater number of receptors for SBA and Dolichos biflorus lectins than T lymphocytes, whilst the opposite was observed for PHA-lectin. However, no significant differences between the two lymphocyte populations were found regarding the receptors for Tetragonolobus purpureus lectin. Among T lymphocyte subpopulations, SBA, Dolichos biflorus and tetragonolobus purpureus lectins appeared to have higher specificity for T gamma lymphocytes and PHA for T mu. PHA-lectin had greater affinity for B lymphocytes, while the rest showed higher affinity for T lymphocytes. The T gamma subpopulation had lower association constant for PHA-lectin than T mu lymphocytes, whilst the contrary was found to be true for the other three lectins.
利用四种¹²⁵I标记的凝集素，对外周血中B淋巴细胞、T淋巴细胞、Tγ淋巴细胞和Tμ淋巴细胞的质膜组成和结构进行了研究。结果显示，B淋巴细胞对大豆凝集素（SBA）和双花扁豆凝集素的受体数量比T淋巴细胞更多，而对植物血凝素（PHA）凝集素的情况则相反。然而，就紫花四棱豆凝集素的受体而言，未发现这两种淋巴细胞群体之间存在显著差异。在T淋巴细胞亚群中，SBA、双花扁豆凝集素和紫花四棱豆凝集素似乎对Tγ淋巴细胞具有更高的特异性，而PHA对Tμ淋巴细胞具有更高的特异性。PHA凝集素对B淋巴细胞具有更高的亲和力，而其余凝集素对T淋巴细胞显示出更高的亲和力。Tγ亚群对PHA凝集素的结合常数低于Tμ淋巴细胞，而对于其他三种凝集素则情况相反。
