Eilon G, Trummel C, Kream B, Viola M V
Cancer Res. 1984 Jan;44(1):209-14.
The FM-2 cell line is a cloned, immortalized cell line derived from a human osteosarcoma. Conditioned medium from FM-2 cultures contains a factor which stimulates calcium mobilization from fetal rat bone organ cultures. Treated bones contain increased numbers of osteoclasts and decreased bone matrix. This factor has a molecular weight of approximately 29,000 as determined by gel filtration. Its biological activity is dependent on a protein moiety and is completely inhibited by calcitonin. Its synthesis by the FM-2 line is dependent on cell density and replenishment of fresh medium. This factor is not parathyroid hormone, a vitamin D metabolite, prostaglandin E, epidermal growth factor, or osteoclast-activating factor, all of which have bone-resorbing activities. Also, FM-2-conditioned medium inhibits collagen synthesis in fetal rat calvaria cells and decreases alkaline phosphatase levels in an osteoblastic cell line, and these two properties coelute with the calcium-mobilizing factor from a hydroxylapatite column. These biological products, synthesized by a cell line derived from a tumor, may represent physiological factors normally synthesized by a subpopulation of bone cells.
FM - 2细胞系是一种源自人骨肉瘤的克隆永生细胞系。FM - 2培养物的条件培养基含有一种能刺激胎鼠骨器官培养物中钙动员的因子。经处理的骨骼中破骨细胞数量增加，骨基质减少。通过凝胶过滤测定，该因子的分子量约为29,000。其生物活性依赖于蛋白质部分，并且被降钙素完全抑制。FM - 2细胞系对其合成依赖于细胞密度和新鲜培养基的补充。该因子不是甲状旁腺激素、维生素D代谢物、前列腺素E、表皮生长因子或破骨细胞激活因子，所有这些都具有骨吸收活性。此外，FM - 2条件培养基抑制胎鼠颅骨细胞中的胶原蛋白合成，并降低成骨细胞系中的碱性磷酸酶水平，这两种特性与来自羟基磷灰石柱的钙动员因子共洗脱。这些由肿瘤衍生的细胞系合成的生物产物可能代表正常情况下由骨细胞亚群合成的生理因子。
