Steinberg R H, Linsenmeier R A, Griff E R
Vision Res. 1983;23(11):1315-23. doi: 10.1016/0042-6989(83)90107-4.
This paper summarizes our findings on light-evoked changes in retinal pigment epithelial cell (RPE) membrane potentials. Experiments were performed on the eye of the anesthetized or decerebrate cat and on isolated tissues from the eyes of a lizard, Gekko gekko, and a frog, Rana catesbeiana. In cat, as was previously shown, the RPE apical membrane potential responds to changes in [K+]0 in the subretinal space. At the onset of illumination it hyperpolarizes to a peak at 4.0 sec as [K+]0 decreases. The next RPE response is a hyperpolarization of the basal membrane that peaks at 20 sec and is also dependent on the decrease in subretinal [K+]0. The last and slowest response is a depolarization of the basal membrane that peaks at 300 sec, and is not obviously associated with K+ changes. The same responses also appear in gecko at a slower time-course, but only the apical-membrane K+-response is present in frog. The three responses also are associated with changes of the opposite polarity at the offset of illumination. These changes in membrane potential are the origin, respectively, of the RPE component of the ERG c-wave, the fast oscillation, and the light peak (slow oscillation).
本文总结了我们关于光诱发视网膜色素上皮细胞（RPE）膜电位变化的研究结果。实验在麻醉或去脑猫的眼睛以及从壁虎（蛤蚧）和牛蛙的眼睛分离出的组织上进行。在猫身上，如先前所示，RPE顶端膜电位对视网膜下间隙中[K⁺]₀的变化有反应。光照开始时，随着[K⁺]₀降低，它超极化至4.0秒时达到峰值。RPE的下一个反应是基底膜超极化，在20秒时达到峰值，并且同样依赖于视网膜下[K⁺]₀的降低。最后且最慢的反应是基底膜去极化，在300秒时达到峰值，并且与K⁺变化没有明显关联。相同的反应在壁虎身上也出现，但时间进程较慢，而在青蛙身上仅存在顶端膜K⁺反应。这三种反应在光照结束时也与相反极性的变化相关。这些膜电位变化分别是视网膜电图c波的RPE成分、快速振荡和光峰（慢振荡）的起源。
