[利用麦胚凝集素-琼脂糖分离妊娠特异性蛋白]
[Use of Wheat Germ Lectin-Sepharose for isolating pregnancy-specific protein].
作者信息
Kalashnikov V V, Sokolov A V, Falaleeva D M, Iakobidze V E
出版信息
Biull Eksp Biol Med. 1983 Dec;96(12):118-20.
PMID:6607075
Abstract
Specific pregnancy protein was obtained from retroplacental blood serum by saline fractionation, affinity chromatography on Wheat germ Lectin-Sepharose, gel filtration on Sephadex G-200, hydrophobic chromatography on Phenyl-Sepharose, and ion exchange chromatography on DEAE-Sephacel. The purified preparation of specific pregnancy protein is highly suitable for preparing antisera and the development of immuno- and radioassays of the protein in question.
摘要
通过盐析从胎盘后血清中获得特异性妊娠蛋白，然后依次经麦胚凝集素-琼脂糖亲和层析、葡聚糖凝胶G-200凝胶过滤、苯基-琼脂糖疏水层析以及二乙氨基乙基-琼脂糖离子交换层析进行纯化。纯化后的特异性妊娠蛋白制剂非常适合用于制备抗血清以及开发针对该蛋白的免疫分析和放射免疫分析。
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