Mal'tsev A V, Riabov V T
Vopr Med Khim. 1984 Mar-Apr;30(2):63-7.
Using analytical PAAG-electrophoresis two specific proteins, D1 and D2, were found in decidual tissue of pregnant women (6-12 weeks). These proteins were isolated and purified by means of ammonium sulfate saturation, chromatography on Sepharose 6B and ion exchange chromatography on DEAE 52 cellulose. The degree of purification of both D1 and D2 proteins was 100% and 81%, respectively. The both proteins were free of DNA, RNA and polysaccharides. The pI values of D1 and D2 proteins were at pH 5.6-6.0. According to gel electrophoresis on PAA-SDS-Na and gel filtration on Sepharose 6B, molecular weight of D1 protein was about 50,000-55,000 and of D2 protein--150,000-160,000 and 300,000-320,000, respectively. The specific decidual proteins D1 and D2 appear to be distinct from the so-called "pregnancy proteins" described previously.
采用分析性聚丙烯酰胺凝胶电泳法，在怀孕6至12周孕妇的蜕膜组织中发现了两种特异性蛋白质D1和D2。通过硫酸铵饱和度、Sepharose 6B柱层析以及DEAE 52纤维素离子交换层析法对这些蛋白质进行了分离和纯化。D1和D2两种蛋白质的纯化程度分别为100%和81%。这两种蛋白质均不含DNA、RNA和多糖。D1和D2蛋白质的等电点在pH 5.6 - 6.0之间。根据PAA - SDS - Na凝胶电泳和Sepharose 6B凝胶过滤结果，D1蛋白质的分子量约为50,000 - 55,000，D2蛋白质的分子量分别为150,000 - 160,000和300,000 - 320,000。特异性蜕膜蛋白质D1和D2似乎与先前描述的所谓“妊娠蛋白”不同。
