Tanase H, Matsunuma N, Suzuki Y
Jikken Dobutsu. 1983 Oct;32(4):201-8.
The present study is an attempt to utilize hybrids among several inbred strains of rats as useful animals for the studies of effectiveness and toxicology on drugs., Four-way crosses were made among the LEW, WM, F344 and DRY strains of rats, and their characteristics were examined. From the breeding data of diallel crosses among these four strains and reciprocal crosses among their F1 hybrids, the mating type indicating the highest reproductivity was (LEW X WM) F1 X (F344 X DRY) F1. These four-way crosses were designated as LWFD. The reproductivity of this mating type was exceedingly higher than those of four strains. In order to examine the susceptibility to thiamine hydrochloride, the acute toxicity test was practiced in inbred strains, F1 hybrids and four-way crosses. As a result, in spite of highly heterogeneous population, the LWFD did not show a peculiar response in comparison with four strains and their F1 hybrids. Furthermore, hematological and clinico-biochemical values of the LWFD did not show a large variability as presumed. From these results, it is suggested that hybrids such as four-way crosses among inbred strains can be used as useful animals for the studies of effectiveness and toxicology on drugs.
本研究旨在利用几种近交系大鼠之间的杂种作为研究药物有效性和毒理学的实验动物。对LEW、WM、F344和DRY品系的大鼠进行了四系杂交，并对其特性进行了研究。根据这四个品系间双列杂交和其F1杂种间正反交的繁殖数据，繁殖力最高的交配类型为（LEW×WM）F1×（F344×DRY）F1。这些四系杂交被命名为LWFD。这种交配类型的繁殖力远高于这四个品系。为了检测对盐酸硫胺的敏感性，在近交系、F1杂种和四系杂交后代中进行了急性毒性试验。结果表明，尽管LWFD群体高度异质，但与四个品系及其F1杂种相比，并未表现出特殊反应。此外，LWFD的血液学和临床生化指标也没有像预期的那样表现出很大的变异性。根据这些结果，提示近交系间的四系杂交等杂种可作为研究药物有效性和毒理学的有用实验动物。
