第三种补体因子（C3）及其体内裂解产物：与凝集素的相互作用以及用聚乙二醇沉淀

The third complement factor (C3) and its in vivo cleavage products: interaction with lectins and precipitation with polyethylene glycol.

作者信息

Teisner B, Hau J, Svehag S E, Brandslund I, Bøg-Hansen T C

出版信息

J Immunol Methods. 1984 Jan 20;66(1):113-8. doi: 10.1016/0022-1759(84)90254-0.

Abstract

Five molecular forms of C3 expressing D but not C epitopes were identified following in vivo activation of the complement system. Examination of concanavalin A (Con-A) reactivity in crossed immunoelectrophoresis revealed that native C3, C3c and the beta mobile form 4 of C3d were completely precipitated by 100 micrograms Con A/cm2. The alpha-1 mobile form 1 of C3d did not interact with Con A, whereas the alpha-2 mobile forms 2 and 3 were retarded in electrophoretic migration by Con A. Native C3, C3c, and forms 4 and 5 of C3d were precipitated by 12% (w/v) polyethylene glycol (PEG). Form 1 of C3d was soluble in these PEG concentrations, whereas forms 2 and 3 were partially precipitated.

摘要

在补体系统的体内激活后，鉴定出了五种表达D表位而非C表位的C3分子形式。在交叉免疫电泳中对伴刀豆球蛋白A（Con-A）反应性的检测显示，天然C3、C3c和C3d的β移动形式4被100微克Con A/cm²完全沉淀。C3d的α-1移动形式1不与Con A相互作用，而C3d的α-2移动形式2和3在电泳迁移中被Con A阻滞。天然C3、C3c以及C3d的形式4和5被12%（w/v）聚乙二醇（PEG）沉淀。C3d的形式1在这些PEG浓度下可溶，而形式2和3则部分沉淀。