Tanaka N, Ichino Y, Ikawa T, Ishikawa T
Acta Otolaryngol Suppl. 1983;401:68-74. doi: 10.3109/00016488309121160.
The experiment was performed in order to approach the question whether denatured epithelial debris in the tonsillar crypts could be an antigenic substance which might be responsible for inducing autoallergic reactions in the skin. Identical antigenicity of the tonsillar epithelium and of the skin was determined by immunofluorescence study. Then heat-denatured skin extract was used as a model system for studying the antigenicity of denatured epithelial debris. A blastoid transformation study of tonsil and peripheral blood lymphocytes obtained from patients with pustulosis palmaris et plantaris and control individuals was carried out by stimulating with heat-denatured skin extract. Tonsil lymphocytes responded well to skin extract, but peripheral blood lymphocytes scarcely responded at all. Skin extract induced transformation of tonsil lymphocytes occurred in the T-cell fraction, but not in the B-cell fraction. The results indicate that denatured tonsillar epithelium can induce an immune response of tonsil lymphocytes as autoantigen, and that the blastogenesis observed was dependent upon T cells.
进行该实验是为了探讨扁桃体隐窝中的变性上皮碎片是否可能是一种抗原物质，该物质可能是引发皮肤自身过敏反应的原因。通过免疫荧光研究确定了扁桃体上皮和皮肤具有相同的抗原性。然后，将热变性皮肤提取物用作研究变性上皮碎片抗原性的模型系统。通过用热变性皮肤提取物刺激，对掌跖脓疱病患者和对照个体的扁桃体和外周血淋巴细胞进行了类 blastoid 转化研究。扁桃体淋巴细胞对皮肤提取物反应良好，但外周血淋巴细胞几乎没有反应。皮肤提取物诱导的扁桃体淋巴细胞转化发生在 T 细胞部分，而不是 B 细胞部分。结果表明，变性扁桃体上皮可作为自身抗原诱导扁桃体淋巴细胞的免疫反应，并且观察到的 blastogenesis 依赖于 T 细胞。
