Standefer J C, Anderson R E, Wilder M, Martin J
Am J Pathol. 1984 Feb;114(2):301-8.
Low doses of radiation reduce the rate and magnitude of fluorescent probe interaction with spleen cells. The uptake of 8-anilino-naphthalene sulfonic acid (ANS), but not diphenyl-hexatriene (DPH), is reduced in a dose-dependent fashion in cells that receive 25-100 rads. The effects of irradiation are most evident in a "medium" fluorescent subpopulation with no effect observed for a "dim" subpopulation. Splenic lymphocytes enriched for T cells show lower uptake of ANS, compared with a B-cell-enriched population. In addition, the normal decrease in ANS fluorescence polarization that occurs during the initial 10 minutes of probe interaction is attenuated in irradiated cells. These findings are consistent with the notion that low doses of radiation limit the penetration of ANS, but not DPH, into a less restrictive microenvironment within the plasma membranes of T, but not B, cells.
低剂量辐射可降低荧光探针与脾细胞相互作用的速率和程度。在接受25 - 100拉德辐射的细胞中，8 - 苯胺基萘磺酸（ANS）的摄取量呈剂量依赖性降低，而二苯基己三烯（DPH）的摄取量则未受影响。辐射效应在“中等”荧光亚群中最为明显，而“暗淡”亚群未观察到影响。与富含B细胞的群体相比，富含T细胞的脾淋巴细胞对ANS的摄取量较低。此外，在探针相互作用的最初10分钟内正常发生的ANS荧光偏振降低在受辐射细胞中减弱。这些发现与低剂量辐射限制ANS（而非DPH）进入T细胞（而非B细胞）质膜内限制较少的微环境这一观点一致。
