Merry A H, Thomson E E, Rawlinson V I, Stratton F
Clin Lab Haematol. 1983;5(4):387-97. doi: 10.1111/j.1365-2257.1983.tb00512.x.
A sensitive method is described for the quantification of C3 fragments on erythrocytes. A radiolabelled monoclonal antibody, was used which was directed against a C3d determinant on all forms of cell bound C3. The number of C3 molecules on normal erythrocytes was estimated to be 420 +/- 140. The strength of the antiglobulin test increased from negative to 5+ over a range of only 850 C3 molecules (400-1250). A blood donor with a positive direct antiglobulin test was found to have 4800 molecules per cell whereas three cases of cold haemagglutinin disease with active haemolysis had from 16 000 to 52 020 C3 molecules per cell. This test has an application in the testing of acquired haemolytic anaemia cases with a positive direct antiglobulin test with C3 bound to the cells and in the standardization of sensitized cells used for testing antiglobulin reagents by various serological techniques.
本文描述了一种用于定量红细胞上C3片段的灵敏方法。使用了一种放射性标记的单克隆抗体，该抗体针对所有形式的细胞结合C3上的C3d决定簇。正常红细胞上C3分子的数量估计为420±140。抗球蛋白试验的强度在仅850个C3分子（400 - 1250）的范围内从阴性增加到5+。发现一名直接抗球蛋白试验阳性的献血者每个细胞有4800个分子，而三例伴有活跃溶血的冷凝集素病患者每个细胞有16000至52020个C3分子。该试验可应用于检测直接抗球蛋白试验阳性且细胞结合有C3的获得性溶血性贫血病例，以及用于通过各种血清学技术检测抗球蛋白试剂的致敏细胞的标准化。
