人脐带条件培养基：人CFU-G的一种刺激物。
Human umbilical cord conditioned medium: a stimulus for human CFU-G.
作者信息
Irvine A E, Morris T C, Kennedy H, Wisdom G B, Bridges J M
出版信息
Exp Hematol. 1984 Jan;12(1):19-24.
Segments of human umbilical cord have been used to prepare a highly active conditioned medium (HUCCM) for in vitro human granulopoiesis and have been compared with human placenta conditioned medium (HPCM) and feeder layers. The HUCCM produced more colonies on day 7 than HPCM (P less than 0.001), and colonies consisted almost exclusively (over 98%) of granulocytic cells by both morphological and cytochemical analysis. The HUCCM-stimulated colonies have a short culture life, the number of colonies being maximal on day 7 of culture with few colonies remaining on day 10. The main source of the colony-stimulating activity in the umbilical cord was found in the Wharton's Jelly component.
人脐带片段已被用于制备用于体外人粒细胞生成的高活性条件培养基（HUCCM），并已与人类胎盘条件培养基（HPCM）和饲养层进行了比较。在第7天，HUCCM产生的集落比HPCM更多（P小于0.001），并且通过形态学和细胞化学分析，集落几乎完全（超过98%）由粒细胞组成。HUCCM刺激的集落培养寿命较短，在培养第7天集落数量最多，到第10天几乎没有集落残留。脐带中集落刺激活性的主要来源存在于华通胶成分中。
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