Schlunk T, Schleyer M
Exp Hematol. 1980 Feb;8(2):179-84.
The growth of human granulopoietic progenitor cells (CFUc) in vitro is stimulated by supernatants of human placental tissue cultures. The placenta culture was the subject of systematic experiments with the aim of improving the concentration of colony-stimulating activity (CSA) in the crude material. Different culture parameters were varied and analyzed separately on the basis of dose-response studies. Maximum levels of CSA in human placental conditioned medium (HPCM) were obtained from 7-day cultures with a tissue-to-medium ratio of 1 to 20 and a depth of the culture fluid of 3 mm. Moreover, these modifications of the placenta culture resulted in a considerable reduction of inhibitors. HPCM stimulation of colony growth in agar unfailingly equaled feeder layer stimulation both in terms of colony number and size, each placenta producing enough material for maximum stimulation of 30,000 cultures.
人胎盘组织培养上清液可刺激人粒细胞祖细胞（CFUc）的体外生长。胎盘培养是系统实验的研究对象，目的是提高粗提物中集落刺激活性（CSA）的浓度。基于剂量反应研究，分别对不同的培养参数进行了改变和分析。人胎盘条件培养基（HPCM）中CSA的最高水平是通过组织与培养基比例为1比20、培养液深度为3毫米的7天培养获得的。此外，胎盘培养的这些改变导致抑制剂显著减少。就集落数量和大小而言，HPCM对琼脂中集落生长的刺激始终等同于饲养层刺激，每个胎盘产生的物质足以最大程度地刺激30000个培养物。
